I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as. ![]() I confirm that all necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived, and that any patient/participant/sample identifiers included were not known to anyone (e.g., hospital staff, patients or participants themselves) outside the research group so cannot be used to identify individuals. The details of the IRB/oversight body that provided approval or exemption for the research described are given below:Įthical approval was granted by the Cantonal Ethical Commission for Research, Canton of Bern, Switzerland (GSI-KEK, BASEC-Nr 2022-00597) to sequence and genomically compare SARS-CoV-2 isolates from samples previously screened and submitted to IFIK for viral diagnosis by treating physicians. I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. 40B2-0_203615) and the Multidisciplinary Center for Infectious Diseases, Bern, Switzerland. Swiss National Science Foundation - Bridge Discovery (grant no. The authors have declared no competing interest. The new approach may have applications beyond SARS-CoV-2 sequencing for other DNA or RNA pathogens in clinical samples. It provides complete and accurate genome sequence reconstruction within seven hours at a competitive cost. Using clinical samples positive for SARS-CoV-2 during the Omicron wave, we demonstrate how the method leads to up to >100x coverage of the full genome sequences of the target organism as compared to standard shotgun metatranscriptomics approach. ![]() ![]() Here, we present a new approach, called NASCarD (Nanopore adaptive sampling with carrier DNA), which allows low amount of nucleic acids to be sequenced while selectively enriching for sequences of interest, hence limiting the production of non-target sequences. The rapid, near-full-length sequencing of the viral genome is commonly enabled by high-throughput sequencing of PCR amplicons derived from cDNA molecules. ![]() Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide.
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